2.7.1.173 native enzyme partially by ammonium sulfate fractionation 2.7.1.173 recombinant His-tagged NRK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 2.7.1.173 recombinant Nrk1 and Nrk2 from Escherichia coli by metal chelate affinity chromatography 2.7.1.173 recombinant Nrk2 from Escherichia coli by metal chelate affinity chromatography