2.4.2.6 - 638040, 638041, 638046, 704429, 736587 2.4.2.6 ammonium sulfate precipitation and Sephacryl S-200 gel filtration 737137 2.4.2.6 Bio-Scale Mini UNOsphere Q column chromatography, and Superose 12 gel filtration 736846 2.4.2.6 DRTase I and DRTAse II 638051 2.4.2.6 preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20°C in 50% glycerol 691053 2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 759063 2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 759340 2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and preparative gel fitration 758681 2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and tag cleavage through thrombin 758927 2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, method optimization for LaNDT 759530 2.4.2.6 recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli to homogeneity by nickel affinity chromatography and gel filtration 675307 2.4.2.6 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, preparative gel fitration, dialysis, and ultrafiltration 758684 2.4.2.6 recombinant unmodified enzyme from Escherichia coli strain Bli5 (BL21 (DE3) pDIA17) by anion exchange chromatography and gel filtration, recombinant His-tagged enzyme by nickel affinity chromatography, over 95% purity 674900