2.3.1.47 - 755855 2.3.1.47 from Escherichia coli DH5alpha, ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, ion exchange chromatography 718696 2.3.1.47 Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography 719127 2.3.1.47 Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, gel filtration 719127 2.3.1.47 Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, heat treatment with centrifugation 719127 2.3.1.47 Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, ion exchange chromatography 719127 2.3.1.47 immobilized metal ion affinity chromatography (Ni2+), His-tag cleavage, immobilized metal ion affinity chromatography (Ni2+), gel filtration 719127 2.3.1.47 maltose binding protein affinity chromatography 720623 2.3.1.47 native enzyme partially by subcellular fractionation, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 676580 2.3.1.47 Ni-NTA column chromatography 755855 2.3.1.47 partial 487051, 487052 2.3.1.47 recombinant enzyme from Escherichia coli strain BL21-Gold (DE) 736946 2.3.1.47 recombinant from E. coli 487057, 487058 2.3.1.47 recombinant from E. coli overproducing strain 487048, 487052, 487053, 487054, 487055 2.3.1.47 recombinant from overexpressing E. coli strain HMS174 (DE3) 487056 2.3.1.47 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affiinity chromatography and gel filtration to homogeneity 674626 2.3.1.47 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinit chromatography, desalting gel filtration, and anion exchange chromatography followed by gel filtration, to over 95% purity 735394