2.3.1.142 gastric mucosa, solubilized with Triton X-100, affinity chromatography 2.3.1.142 partial, 95fold from liver by preparation of plasma membrane fraction, solubilization with 0.15% Triton X-100, and sequential chromatography including affinity chromatography on a cerulin-based palmitoylation inhibitor ligand 2.3.1.142 recombinant His-tagged Erf2 with fused FLAG-tagged Erf4 from strain RJY1827, solubilization by 0.8% dodecylmaltoside and urea, and ultracentrifugation, purification by nickel affinity chromatography and desalting gel filtration, recombinant FLAG-tagged Erf2 by the same method 2.3.1.142 solubilized with Triton X-100 or Nonidet P-40