2.1.1.202 recombinant His6-tagged enzyme HNSun6, hNSun6 and hNSun6-C326A-RNA complexes by nickel affinity chromatography 2.1.1.202 recombinant MJ0026 from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by heat treatment, anion exchange and hydroxyapatite chromatography, and dialysis 2.1.1.202 recombinant Ybl024p is purified to apparent homogeneity by one-step affinity chromatography on Ni21-NTA-agarose column