2.1.1.158 native enzyme 667105 2.1.1.158 native enzyme 9fold from young leaves by 3 steps of ion exchange chromatography, and gel filtration 670509 2.1.1.158 native enzyme partially from young leaves by ammonium sulfate fractionation, gel filtration, and ultrafiltration 667116 2.1.1.158 recombinant His-tagged XMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration 667101 2.1.1.158 using the His-tagged affinity chromatography 684218