1.8.1.8 - 1.8.1.8 HisTrap column chromatography and HiLoad Superdex 16/60 75 gel filtration 1.8.1.8 Ni2+-NTA affinity chromatography 1.8.1.8 recombinant enzyme 1.8.1.8 recombinant His-tagged enzyme from Escherichia coli 1.8.1.8 Superdex 200 gel filtration