1.14.14.3 - 348579, 348582, 348594, 348599, 348604, 348606, 348607, 348608 1.14.14.3 affinity methods 348560 1.14.14.3 DEAE-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography 728435 1.14.14.3 HPLC 348561 1.14.14.3 nickel affinity column 698992 1.14.14.3 on a nickel affinity column, to more than 90% purity 702318 1.14.14.3 on nickel affinity column, to more than 90% purity 704579 1.14.14.3 preparation of enzyme with modified subunits 348584 1.14.14.3 preparation of subunits 348583 1.14.14.3 recombinant enzyme from Escherichia coli 658147 1.14.14.3 recombinant enzyme from Escherichia coli strain JM101 to over 95% purity 671610 1.14.14.3 recombinant wild-type and mutant C106A enzymes from Escherichia coli strain JM101 to over 95% purity 658050 1.14.14.3 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 658060 1.14.14.3 recombinant wild-type and mutant enzymes from Escherichia coli strain JM101 to homogeneity 671978 1.14.14.3 recombinant wild-type and mutant enzymes from Escherichia coli strain JM109 to over 85% homogeneity 657923