1.14.11.26 - 1.14.11.26 anion-exchange chromatography and gel filtration 1.14.11.26 DAOC hydroxylase of Streptomyces clavuligerus is purified to near homogeneity by anion-exchange chromatography, ammonium sulphate precipitation and gel filtration 1.14.11.26 expression in Escherichia coli, overexpression as an insoluble and inactive enzyme, elaboration of refolding scheme resulting in highly active and moderately stable enzyme 1.14.11.26 native enzyme, partial, recombinant enzyme, to near homogenity 1.14.11.26 partial