1.13.11.4 - 1.13.11.4 ammonium sulfate fractionation, DEAE-Sepharose fast flow column chromatography 1.13.11.4 GSTrap fast flow column chromatography 1.13.11.4 His-Bind protein chromatography 1.13.11.4 HiTrapQ column chromatography 1.13.11.4 Ni-NTA column chromatography 1.13.11.4 nickel-nitriloacetic acid resin column chromatography 1.13.11.4 partial 1.13.11.4 recombinant 1.13.11.4 recombinant enzyme 1.13.11.4 recombinant mutant G106A 1.13.11.4 recombinant Nagl2 and Nagl3 isozymes from Escherichia coli strain BL21(DE3) by gel filtration and sequential anion exchange chromatography 1.13.11.4 recombinant protein from Escherichia coli