1.1.1.B4	-	
1.1.1.B4	native enzyme 226fold to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by gel filtration	
1.1.1.B4	native enzyme by a procedure including RedA-agarose affinity chromatography to electrophoretical homogeneity	
1.1.1.B4	Ni Sepharose column chromatography	
1.1.1.B4	recombinant C-terminally His-tagged mutant I86A from Escherichia coli HB101 (DE3) by nickel affinity chromatography	
1.1.1.B4	recombinant enzyme 11fold to homogeneity from Escherichia coli strain BL21(DE3) by heat denaturation of host proteins, anion exchange chromatography and gel filtration