1.1.1.312 from the cell extract of Escherichia coli, using POROS polyethyleneimine anion-exchange chromatography, POROS quaternized PI anion-exchange chromatography and POROS phenylether hydrophobic-interaction chromatography 1.1.1.312 recombinant enzyme expressed in Escherichia coli 1.1.1.312 using gel filtration on Sephadex G-200