1.1.1.31 - 286544, 286546, 286547, 286548, 286549, 286550, 286551, 655051 1.1.1.31 96fold purified at 4°C by ammonium sulfate fractionation and gel filtration 695710 1.1.1.31 affinity chromatography 670284 1.1.1.31 ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography 695710 1.1.1.31 native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography 695710 1.1.1.31 Ni-NTA column chromatography and Superdex 200 gel filtration 737911 1.1.1.31 Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration 737620 1.1.1.31 Ni-NTA resin column chromatography 739465, 760528