1.1.1.3 - 246383, 246384, 246388, 246389, 246391, 246392, 246395, 246398, 246399 1.1.1.3 native enzyme partially from seeds by ammonium sulfate fractionation and desalting gel filtration 689647 1.1.1.3 overexpressed in Escherichia coli 246402 1.1.1.3 partial 246393 1.1.1.3 recombinant enzyme 662432, 712490 1.1.1.3 recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, dialysis, and ultrafiltration 762453 1.1.1.3 recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 70°C for 3 h, anion exchange chromatography, dialysis, and ultrafiltration 739972 1.1.1.3 recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment, hydrophobic interaction chromatography, gel filtration, hydroxyapatite chromatography, and ultrafiltration 760736 1.1.1.3 recombinant enzyme from Escherichia coli strain Rosetta(DE3)pLys by heat treatment at 70°C for 10 min, gel filtration, hydrophobic interaction and anion exchange chromatography, desalting gel filtration, and ultrafiltration, to homogeneity 761404 1.1.1.3 recombinant His-tagged enzyme from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration 739804 1.1.1.3 recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) to homogeneity by nickel affinity chromatography and two different steps of gel filtration 761687 1.1.1.3 recombinant His-tagged wild-type and mutant enzymes from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration 739791 1.1.1.3 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) codon plus-RIPL by heat treatment at 90°C for 10 min, nickel affinity chromatography, dialysis, gel filtration, and ultrafiltration 741408 1.1.1.3 recombinant separated catalytic domains 654640 1.1.1.3 separation of the two isozymes using Blue-Sepharose chromatography 246382 1.1.1.3 separation of the two isozymes using Matrex Gel Red A affinity chromatography 246382 1.1.1.3 threonine sensitive and threonine insensitive isozymes 246385