3.4.22.53 recombinant enzyme 643968, 644028, 644029 3.4.22.53 recombinant wild-type and mutant enzymes 644019 3.4.22.53 single-step purification of calpain-1, calpain-2, and calpastatin using anion-exchange chromatography 754631 3.4.22.53 wild-type and mutant calpains 644019