1.1.1.3 recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) to homogeneity by nickel affinity chromatography and two different steps of gel filtration 761687 1.1.1.3 recombinant His-tagged wild-type and mutant enzymes from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration 739791 1.1.1.3 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) codon plus-RIPL by heat treatment at 90°C for 10 min, nickel affinity chromatography, dialysis, gel filtration, and ultrafiltration 741408 1.1.1.3 recombinant separated catalytic domains 654640 1.1.1.3 separation of the two isozymes using Blue-Sepharose chromatography 246382 1.1.1.3 separation of the two isozymes using Matrex Gel Red A affinity chromatography 246382 1.1.1.3 threonine sensitive and threonine insensitive isozymes 246385