3.4.24.75 glycoprotein Asn125 is the exact glycosylation site of lysostaphin expressed in CMEC-08-D cells. N-glycosylation at Asn125 of lysostaphin decreases lysostaphin bacteriolytic activity but does not affect its binding ability to Staphylococcus aureus 733095 3.4.24.75 proteolytic modification lysostaphin is secreted as a proenzyme. Proteolytic cleavage of 13 N-terminal tandem repeats generates mature lysostaphin with 2 functionally separable domains 695804 3.4.24.75 proteolytic modification the preprolysostaphin is proteolytically processed to the mature enzyme 670916