2.1.3.1	side-chain modification	97 amino acids deleted of the carboxyl-terminal region of the monomer of the 12S subunit, 46% loss of activity because of a reduces stability of the 12S subunit and a decrease in binding of 6S subunit to the 12 subunit	485830	
2.1.3.1	side-chain modification	a series of truncated forms of the 1.3S subunit is prepared and the effect on the first and the second partial reaction and the overall enzyme reaction measured, result: the sequence 59-78 of 1.3S subunit is necessary for the carboxylation of biotin of the 1.3 S subunit, the inability of the truncated peptides to form enzyme complexes explains the activity loss in the overall reaction	485827	
2.1.3.1	side-chain modification	deletion of residues 10 to 48 of 1.3S subunit, not necessary for catalysis of the partial reaction	485834