1.14.99.54 methylation the enzyme contains an N-methylated histidine at the N-terminus -, 735814 1.14.99.54 glycoprotein intact protein bears three glycan chains: (Man)9(GlcNAc)2, (Man)10(GlcNAc)2 and (Man)11(GlcNAc)2 -, 739826 1.14.99.54 additional information when the three disulfide bridges of the protein are broken, irreversible unfolding occurs and no residual activity can be detected after a heat treatment at 80°C -, 741358 1.14.99.54 glycoprotein protein expressed in Pichia pastoris displays a molecular mass of 50 kDa, and 27 kDa after deglycosylation using PNGase F -, 744804 1.14.99.54 glycoprotein lack of potential N-glycosylation sites, presence of 37 predicted O-linked glycosylation sites. Molecular mass of recombinant protein is 75 kDa by SDS-PAGE, as compared with 32500 predicted 746230