2.3.1.94	analysis	kinetic assay for the megasynthase that stoichiometrically relates NADPH consumption to polyketide production	736307	
2.3.1.94	biotechnology	a derivative of Escherichia coli is genetically engineered to produce 6-deoxyerythronolide B, the macrocyclic core of the antibiotic erythromycin	706754	
2.3.1.94	biotechnology	a new Escherichia coli stain, YW9, is created, featuring a plasmid-free heterologous pathway for the production of the polyketide product 6-deoxyerythronolide B	705513	
2.3.1.94	biotechnology	polyketide synthases synthesize the polyketide cores of biologically active compounds, including natural products that have become important pharmaceuticals, like erythromycin	706566	
2.3.1.94	synthesis	the deoxyerythronolide B synthase genes are functionally expressed in Bacillus subtilis when the native eryAI–III operon is separated into three individual expression cassettes with optimized ribosomal binding sites. A synthesis of 6-deoxyerythronolide B can be detected by using the acetoininducible acoA promoter and a fed-batch simulating EnBase-cultivation strategy. Bacillus subtilis is capable of the secretion of 6-deoxyerythronolide B into the medium. The deletion of the prpBD operon of Bacillus subtilis, responsible for propionyl-CoA utilization, results in a significant increase of the 6-deoxyerythronolide B product yield when exogenous propionate is provided	735515