2.3.1.167	biotechnology	optimizing the semi-biosynthetic method in bacteria potentially provides a practical means of developing commercial-scale production of baccatin III analogs, which serve as key intermediates in the semi-synthesis of second-generation taxols	
2.3.1.167	pharmacology	use in synthesis of taxol for anticnacer treatment	
2.3.1.167	pharmacology	key enzyme in the biosynthesis of the anticancer drug paclitaxel, which catalyses the formation of baccatin III from 10-deacetylbaccatin III	
2.3.1.167	pharmacology	paclitaxel is a type of broad-spectrum anticancer drug in short supply. The price of acetyl-CoA, which is the acetyl group donor for the enzymatic synthesis of the intermediate, baccatin III, is the bottleneck of the mass production of paclitaxel. The study reports that N-acetyl-D-glucosamineas an acetyl group donor can substantially reduce the cost of production	
2.3.1.167	synthesis	bioengineered Escherichia coli cells synthesize nonnatural 10-deacetyl-10-acylbaccatin III derivatives as potential precursors of second-generation Taxol derivatives using the enzyme in concert with acyl-CoA transferases, determination of optimal alkanoic acid concentration for in vivo production of baccatin III analogues, overview	
2.3.1.167	synthesis	synthesis of baccatin III in Escherichia coli producing endogenous acetyl-CoA and overexpressing recombinant 10-deacetylbaccatin III 10-O-acetyltransferase. Use of enzyme to couple unnatural acyl-CoA analogues various amino and/or hydroxyl acceptors	
2.3.1.167	synthesis	transgenic Flammulina velutipes expressing the DBAT gene is able to produce baccatin III, an advanced precursor of paclitaxel	
2.3.1.167	synthesis	key enzyme in the biosynthesis of the anticancer drug paclitaxel, which catalyses the formation of baccatin III from 10-deacetylbaccatin III	
2.3.1.167	synthesis	mutant enzyme G38R/F301V with a catalytic efficiency approximately six times higher than that of the wild-type is combined with a beta-xylosidase to obtain an in vitro one-pot conversion of 7-beta-xylosyl-10-deacetyltaxol to Taxol yielding 0.64 mg/mlx02taxol in 50 ml at 15 h. This approach represents a promising environmentally friendly alternative for Taxol production from an abundant analogue	
2.3.1.167	synthesis	paclitaxel is a type of broad-spectrum anticancer drug in short supply. The price of acetyl-CoA, which is the acetyl group donor for the enzymatic synthesis of the intermediate, baccatin III, is the bottleneck of the mass production of paclitaxel. The study reports that N-acetyl-D-glucosamineas an acetyl group donor can substantially reduce the cost of production