1.2.1.99 synthesis 3-hydroxypropionic acid is an important compound from which several commodity and specialty chemicals can be generated. The DELTAdhaT mutant (deletion in gene dhaT, encoding NADH-dependent 1,3-propanediol oxidoreductase) overexpressing PuuC has a potential to coproduce two commercially valuable products, 3-hydroxypropionic acid and 1,3-propanediol 1.2.1.99 synthesis 3-hydroxypropionic acid is an important compound from which several commodity and specialty chemicals can be generated. The recombinant strain SH254 producing 3-hydroxypropionate from glycerol is developed by cloning of dhaB and aldH genes in Escherichia coli BL21 under different regulatory promoters. The recombinant SH254 can accumulate 3-hydroxypropionic acid from glycerol at 6.5 mmol/l in 30 h 1.2.1.99 synthesis PuuC is an active enzyme that can be utilized effectively for several purposes, including biological production of 3-hydroxypropionate 1.2.1.99 synthesis 3-hydroxypropionic acid is an important platform chemical that can be used to synthesize a range of chemical compounds, such as acrylic acid, 1,3-propanediol, methyl acrylate, malonic acid, acrylamide, and hydroxyamides