1.14.14.3 agriculture engineering of broad-host-range Erwinia amylovora virus Y2 to enhance its killing activity and for use as a luciferase reporter phage. The reporter phage Y2::luxAB transduces bacterial luciferase into host cells and induces synthesis of large amounts of a LuxAB luciferase fusion. After the addition of aldehyde substrate, bioluminescence can be monitored, and enables rapid and specific detection of low numbers of viable bacteria 744110 1.14.14.3 agriculture optimization of fused luxAB expression, quantum yield and application as a reporter gene in plant protoplasts. Luciferase activity is dramatically increased upon use of the optimized gene and the 35S promoter compared to the original luxAB in Arabidopsis and maize cells 746250 1.14.14.3 analysis a minimized cascade for Lux with greater ease of use, utilizes a chemoenzymatic reaction with biomimetic nicotinamide 1-benzyl-1,4-dihydronicotinamide in place of the flavin reductase reaction in the Lux system. The minimized cascade reaction can be applied to monitor bioluminescenceof the Lux reporter in eukaryotic cells effectively, and can achieve higher efficiencies than the system with flavin reductase 765342 1.14.14.3 analysis bioluminescent assay based on a system of coupled enzymatic reactions catalyzed by bacterial luciferase and NADH:FMN-oxidoreductase to monitor toxicity and antioxidant activity of bioactive compounds such as fullerenols, perspective pharmaceutical agents, nanosized particles, water-soluble polyhydroxylated fullerene-60 derivatives. Fullerenols suppress bioluminescent intensity at concentrations above 0.01 g/l and above 0.001 g/l for C60O2-4(OH)20-24 and Fe0.5C60(OH)xOy, respectively 745976 1.14.14.3 analysis enzyme can be used to monitor changes in gene expression as a reporter system in slow-growing mycobacteria, i.e. Mycobacterium tuberculosis strain H37Ra, determination of recombinant enzyme decay rate 658778 1.14.14.3 analysis expression of the bacterial luciferase system in mammalian cells for generation of bioreporters for in vivo monitoring and diagnostics technology, method evaluation and optimization, overview 675217 1.14.14.3 analysis fusion of circularly permuted Venus, a bright variant of yellow fluorescent protein, to the C-terminus of subunit LuxB to induce bioluminescence resonance energy transfer (BRET). By using decanal as added substrate, color change and ten-times enhancement of brightness is achieved in Escherichia coli upon expression. Expression of the Venus-fused luciferase in human embryonic kidney cell lines or in Nicotiana benthamiana leaves together with the substrate biosynthesis-related genes (luxC, luxD and luxE) enhances the autonomous bioluminescence 765786 1.14.14.3 analysis high-throughput, homogeneous, bioluminescent assay for Pseudomonas aeruginosa gyrase inhibitors and other DNA-damaging agents based on a Photorhabdus luminescens luciferase operon transcriptional fusion to a promoter that responds to DNA damage caused by reduced gyrase levels and fluoroquinoline inhibition 680943 1.14.14.3 analysis sensitive and selective bacterial luminescence method for the detection of pyruvic acid based on lactate dehydrogenase and the bacterial luciferase-FMN:NADH oxidoreductase bioluminescence in vitro. NADH involved in the LDH reaction system can be quantitatively analyzed by the bioluminescence system. A good linear relationship between the luminescence intensity and pyruvic acid concentration is observed within the range of 0.00014–0.001 mol/l, and the pyruvic acid detection limit is 0.000085 mol/l 745979 1.14.14.3 analysis the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview -, 711341 1.14.14.3 biotechnology establishment and evaluation of the enzyme used in a luciferase-based reporter system, pPL2lux, harboring the listerial secA and hlyA promoters translationally fused to luxABCDE, overview 671457 1.14.14.3 biotechnology the enzyme and cyanine fluorescent protein are useful dual reporters for the quantitative analysis of the effects of n-dodecyltrimethylammonium bromide on whole cells and intracellular proteins of Pseudomonas putida 726764 1.14.14.3 diagnostics expression of the bacterial luciferase system in mammalian cells for generation of bioreporters for in vivo monitoring and diagnostics technology, method evaluation and optimization, overview 675217 1.14.14.3 medicine expression in Staphylococcus aureus Xen29. In the absence of antibiotics, staphylococcal bioluminescence increases over time until a maximum after ca. 6 h of growth, and subsequently decreases to the detection threshold after 24 h of growth. Up to minimal inhibitory concentrations of the antibiotics vancomycin, ciprofloxacin, erythromycin or chloramphenicol, bioluminescence increases according to a similar pattern up to 6 h of growth, but after 24 h, bioluminescence is higher than in the absence of antibiotics. Antibiotic pressure impacts the relation between bioluminescence per organism and bioluminescence. Under antibiotic pressure, bioluminescence is not controlled by luxA expression but by cofactors impacting the bacterial metabolic activity 745081 1.14.14.3 medicine transient and stable transfection of human kidney, breast cancer, and colorectal cancer cell lines by a codon optimized lux expression cassette using viral 2A elements as linker regions. The expression product produces autobioluminescent phenotypes in all cell lines tested without the induction of cytotoxicity and allows for repeated interrogation of populations and self-directed control of bioluminescent activation with detection limits and EC50 values similar to traditional reporter systems 746267 1.14.14.3 molecular biology enzyme can be used to monitor changes in gene expression as a reporter system in slow-growing mycobacteria, i.e. Mycobacterium tuberculosis strain H37Ra, determination of recombinant enzyme decay rate 658778 1.14.14.3 molecular biology the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview -, 711341 1.14.14.3 synthesis upon expression in Bacillus subtilis cells, luciferase is substantially more thermostable than in Escherichia coli. Thermal inactivation in Bacillus subtilis at 48.5#°C behaves as a first-order reaction. In Escherichia coli, the first order rate constant of the thermal inactivation exceeds that observed in B. subtilis cells 2.9 times. In dnaK-negative strains of Bacillus subtilis, both the rates of thermal inactivation and the efficiency of refolding are similar to that observed in wild-type strains 765657 1.14.14.3 synthesis upon expression in Bacillus subtilis cells, luciferase is substantially more thermostable than in Escherichia coli. Thermal inactivation in the cells of Escherichia coli and Bacillus subtilis may be described by first and third order kinetics, respectively. In dnaK-negative strains of Bacillus subtilis, both the rates of thermal inactivation and the efficiency of refolding are similar to that observed in wild-type strains 765657