2.7.2.7 ATP + butanoate - 2.7.2.7 ATP + isobutanoate - 2.7.2.7 additional information enzyme is involved in production of butyrate 2.7.2.7 additional information the enzyme is not regulated by the end-product, its specific activity is constant during the fermentation 2.7.2.7 additional information semi-automated reverse engineering algorithm. The reconstructed metabolic network was used to create a genome-scale model that correctly characterized the butyrate kinase knock-out and the asolventogenic M5 pSOL1 megaplasmid degenerate strains. Systematic gene knock-out simulations performed to identify a set of genes encoding clostridial enzymes essential for growth in silico.