2.3.1.54 acetyl-CoA + formate - 2.3.1.54 CoA + pyruvate - 2.3.1.54 CoA + pyruvate formate is an important methyl group donor for anabolic pathway through the formation of folate derivates, formate supply improves Streptococcus thermophilus growth in milk 2.3.1.54 CoA + pyruvate metabolic regulation and networking, overview 2.3.1.54 CoA + pyruvate step in the mitochondrial pathway, overview 2.3.1.54 CoA + pyruvate anaerobic growth under nitrogen, 37°C, pH 7.0, extract enzyme reactions measured at room temperature 2.3.1.54 additional information analysis of the cytosolic proteome involving the enzyme, proteome profile 2.3.1.54 additional information hydrogen is formed from pyruvate by multiple parallel pathways, one pathway involves formate as an intermediate, pyruvate-formate lyase, and formate-hydrogen lyase, comprised of HydA hydrogenase and formate dehydrogenase, and a formate-independent pathway involving pyruvate dehydrogenase, the expression of pflB paralleled the expression of hydA and hyaB 2.3.1.54 additional information The product of yfiD gene is similar to pyruvate formate-lyase activase and it has been reported to activate PFL by replacing the glycyl radical domain, overview, the YfiD protein contributes to the pyruvate formate-lyase flux in the Escherichia coli arcA mutant strain, but not in the wild-type strain 2.3.1.54 additional information pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation, molecular modeling of the FocA-PflB complex, the N-terminus of FocA is important for interaction with enzyme PflB