2.1.1.282 additional information bifunctional Trm5a from Pyrococcus abyssi (PaTrm5a) catalyses not only the methylation of N1, but also the further methylation of C7 on 4 demethylwyosine at position 37 to produce isowyosine (EC 2.1.1.228 and EC 2.1.1.282, respectively) 2.1.1.282 S-adenosyl-L-methionine + 4-demethylwyosine - 2.1.1.282 S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine - 2.1.1.282 S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe the enzyme is involved in the biosynthesis of the tricyclic base wybutosine 2.1.1.282 S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe -