2.1.1.225	S-adenosyl-L-methionine + adenosine4 in tRNAHis	the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA	
2.1.1.225	S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)	the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA	
2.1.1.225	S-adenosyl-L-methionine + cytidine4 in tRNAPro	the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA