1.3.99.30 15-cis-phytoene + 5 acceptor a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products 1.3.99.30 15-cis-phytoene + 5 acceptor the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene 1.3.99.30 15-cis-phytoene + acceptor - 1.3.99.30 all-trans-lycopene + acceptor - 1.3.99.30 all-trans-neurosporene + acceptor - 1.3.99.30 all-trans-phytofluene + acceptor - 1.3.99.30 all-trans-zeta-carotene + acceptor -