1.14.19.6 (Z)-9-tetradecenoic acid + ? biosynthetic pathway for producing the sex pheromone component (Z,E)-9,12-tetradecadienyl acetate in moths involves a DELTA12 desaturase 1.14.19.6 1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ - 1.14.19.6 cis-9-octadecenoic acid + reduced acceptor + O2 PtFAD2 is involved in the biosynthesis of eicosapentaenoic acid 1.14.19.6 eicosenoic acid + reduced acceptor + O2 62% conversion 1.14.19.6 additional information oxygen availability alone can regulate de novo DELTA12-desaturase synthesis in Acanthamoeba castellanii and oxygen can limit the activity of preexisting DELTA12-desaturase 1.14.19.6 additional information PtFAD6 is involved in the biosynthesis of hexadecantrienic acid 1.14.19.6 additional information synergistic effect of high-light and low temperature on cell growth of the DELTA12 fatty acid desaturase mutant 1.14.19.6 additional information the main transition in fatty acid metabolism of Acanthamoeba castellanii during batch growth appears to be primarily related to a rapid decline in DELTA12-desaturase activity after 24 h. The resultant large growth-dependent changes in the degree of fatty acid unsaturation would be expected to affect the physical state and/or fluidity of membranes, and may be related to many of the distinctive physiological and biochemical characteristics displayed by Acanthamoeba castellanii in different stages of batch growth 1.14.19.6 oleic acid + ? - 1.14.19.6 oleic acid + ? DELTA12-desaturase mutant shows delayed spore germination, a twofold reduction in growth, a reduced level of conidiation and complete loss of sclerotial development, compared with the wild-type enzyme