3.1.1.45	4-carboxymethylene-but-2-ene-4-olide + H2O	third enzyme of the halocatechol branch of the beta-ketoadipate pathway, catalyses the hydrolysis of both, (E) and (Z) dienelactone to maleyl acetate	
3.1.1.45	4-carboxymethylenebut-2-en-4-olide + H2O	the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds	
3.1.1.45	4-carboxymethylenebut-2-en-4-olide + H2O	dienelactone hydrolase catalyzes a step in the metabolic conversion of chlorocatechols to beta-ketoadipate	
3.1.1.45	4-chloromuconolactone + H2O	-	
3.1.1.45	azilsartan medoxomil + H2O	-	
3.1.1.45	candesartan cilexetil + H2O	-	
3.1.1.45	cis-2-chloro-3-methyldienelactone + H2O	-	
3.1.1.45	cis-2-chloro-5-methyldienelactone + H2O	-	
3.1.1.45	cis-3-chloro-2-methyldienelactone + H2O	-	
3.1.1.45	cis-5-chloro-2-methyldienelactone + H2O	reaction is part of the degradation of dichloromethylcatechols as central intermediates in the degradation of dichlorotoluenes	
3.1.1.45	cis-dienelactone + H2O	-	
3.1.1.45	dienelactone + H2O	-	
3.1.1.45	dienelactone + H2O	enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate	
3.1.1.45	additional information	-	
3.1.1.45	additional information	natural function is not in the degradation of haloaromatic compounds, the normal substrate might well be one of the more complex natural compounds with a dienelactone structure, or it could also be an intermediate of some other metabolic pathway	
3.1.1.45	additional information	it seems likely, that dienelactone hydrolase has the additional function of detoxification of minor amounts of protoanemonin that may be formed during chloroaromatic degradation	
3.1.1.45	additional information	antibiotic protoanemonin is no substrate	
3.1.1.45	additional information	pathway overview	
3.1.1.45	additional information	the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview	
3.1.1.45	additional information	the enzyme is a typical dienelactone hydrolase belonging to alpha/beta hydrolase family and containing a catalytic triad composed of Cys151, Asp198, and His229 in the active site	
3.1.1.45	additional information	the activity with prodrug-type angiotensin II type 1 receptor blocker differs in a tissue-dependent and subcellular-dependent manner, overview	
3.1.1.45	olmesartan medoxomil + H2O	-	
3.1.1.45	trans-2-chloro-3-methyldienelactone + H2O	-	
3.1.1.45	trans-3-chloro-2-methyldienelactone + H2O	-	
3.1.1.45	trans-5-chloro-2-methyldienelactone + H2O	-	
3.1.1.45	trans-dienelactone + H2O	-	
3.1.1.45	trans-dienelactone + H2O	degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview	
3.1.1.45	trans-dienelactone + H2O	specific for the trans-isomer, no activity with the cis-isomer