2.3.1.47 (S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine 37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, 1 mM S-adenosyl-L-methionine, 2 microM (S)-8-amino-7-oxononanoic acid 2.3.1.47 (S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine 37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, varying S-adenosyl-L-methionine, 9 microM (S)-8-amino-7-oxononanoic acid, varying inhibitor concentration 2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine - 2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA 2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine second step in biotin biosynthesis 2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis 2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine first committed step in the biotin biosynthesis pathway 2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine the enzyme catalyzes the first committed step in the four step pathway, the decarboxylative condensation of L-alanine and pimeloyl-CoA to give (7S)-8-amino-7-oxononanoate 2.3.1.47 additional information the enzyme catalyzes the first committed enzyme of the biotin synthesis pathway 2.3.1.47 pimeloyl-CoA + L-alanine - 2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine -