2.1.1.202	additional information	identification of single-nucleotide resolution of cytosine 5-methylation sites in non-coding ribosomal RNAs and transfer RNAs of all three subcellular transcriptomes across six diverse species, overview. The enzyme does not methylate cytosines at structural positions C47, C48, C49 and C72. Identification of modified cytosines in Arabidopsis thaliana nuclear transcribed tRNAs which are dependent on RMTases TRDMT1 and TRM4B. No cytosine 5-methylation sites are detected in Arabidopsis chloroplast or mitochondrial tRNAs, which is in contrast to animal mitochondrial tRNAs	
2.1.1.202	additional information	identification of single-nucleotide resolution of m5C sites in non-coding ribosomal RNAs and transfer RNAs of all three subcellular transcriptomes across six diverse species, overview	
2.1.1.202	additional information	NSUN2 candidate ncRNA targets identified include RNAs with central functions in the processing, folding and modification of other ncRNAs (RPPH1, Y RNA, SCARNA2), RNAs important for protein synthesis and trafficking (5S rRNA and 7SL RNA) and RNAs involved in multidrug resistance and other processes (Vault RNAs). Notably, all of the NSUN2 targets revealed by 5-azacytidine-mediated RNA immunoprecipitation are either transcribed by RNA Pol III in the nucleolus (SCARNA2 excepted), or function in the nucleolus (SCARNA2), where NSUN2 resides	
2.1.1.202	additional information	the enzyme Trm4 fabricate 5-methylcytosine (m5C) in RNA molecules utilizing a dual-cysteine catalytic mechanism	
2.1.1.202	additional information	the iCLIP method identifies tRNA AspGTC, ValAAC, GlyGCC, and LeuCAA as methylation substrates with methylation within the variable arm at cytosines 48, 49, and 50, no additional NSun2 target sites outside the variable arm. vtRNAs are methylation substrates for NSun2, vtRNAs are ncRNAs found as part of the vault ribonucleoprotein complex	
2.1.1.202	S-adenosyl-L-methionine + cytosine in tRNA precursor	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine15 in vtRNA1.3	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine27 in vtRNA1.2	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine27 in vtRNA1.3	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine34 in tRNA	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine34 in tRNA	the enzyme is responsible for complete m5C methylation of yeast tRNA. The frequency of modification depends on the cytosine position in tRNA. At positions 34 and 40, m5C is found only in two yeast tRNAs (tRNALeu (CUA) and tRNAPhe (GAA), respectively), whereas most other elongator yeast tRNAs bear either m5C48 or m5C49, but never both in the same tRNA molecule	
2.1.1.202	S-adenosyl-L-methionine + cytosine34 in tRNA	cf. EC 2.1.1.203	
2.1.1.202	S-adenosyl-L-methionine + cytosine34 in tRNALeu precursor	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine34 in tRNAPro(CGG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine4( in tRNAPro(AGG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine40 in tRNA	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine40 in tRNA	the enzyme is responsible for complete m5C methylation of yeast tRNA. The frequency of modification depends on the cytosine position in tRNA. At positions 34 and 40, m5C is found only in two yeast tRNAs (tRNALeu (CUA) and tRNAPhe (GAA), respectively), whereas most other elongator yeast tRNAs bear either m5C48 or m5C49, but never both in the same tRNA molecule	
2.1.1.202	S-adenosyl-L-methionine + cytosine40 in tRNA precursor	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine40 in tRNAGly precursor	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNA	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNA	the enzyme is responsible for complete m5C methylation of yeast tRNA. The frequency of modification depends on the cytosine position in tRNA. At positions 34 and 40, m5C is found only in two yeast tRNAs (tRNALeu (CUA) and tRNAPhe (GAA), respectively), whereas most other elongator yeast tRNAs bear either m5C48 or m5C49, but never both in the same tRNA molecule	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAAla(AGC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAAla(CGC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAAla(UGC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAAsp precursor	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAAsp(GUC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAAspGTC	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAGln(CUG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAGln(UUG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAGlu(CUC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAGlu(UUC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAGly precursor	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAGly(CCC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAGly(GCC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAGly(UCC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAGlyGCC	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAHis	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAHis(GUG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAIle(AAU)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNALeu(AAG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNALeu(CAA)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNALeu(CAA) precursor	NSUN2-mediated methylation of C34 of tRNALeu(CAA) has been shown to occur exclusively on intron-containing tRNA precursors	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNALeu(CAG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNALeu(UAA)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNALeu(UAG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNALeuCAA	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNALys(CUU)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNALys(UUU)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAMet(CAU)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAPhe(GAA)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAPro(CGG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAPro(UGG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNASer(AGA)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNASer(CGA)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNASer(GCU)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNASer(UGA)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAThr(AGU)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAThr(CGT)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAThr(UGU)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNATyr(GUA)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAVal(AAC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAVal(CAC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine48 in tRNAVal(UAC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNA	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNA	the enzyme is responsible for complete m5C methylation of yeast tRNA. The frequency of modification depends on the cytosine position in tRNA. At positions 34 and 40, m5C is found only in two yeast tRNAs (tRNALeu (CUA) and tRNAPhe (GAA), respectively), whereas most other elongator yeast tRNAs bear either m5C48 or m5C49, but never both in the same tRNA molecule	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAasp precursor	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAAsp(GUC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAAspGTC	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAGln(CUG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAGln(UUG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAGlu(CUC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAGlu(UUC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAGly precursor	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAGly(CCC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAGly(GCC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAGly(UCC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAGlyGCC	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNALeuCAA	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNALys(UUU)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAPhe(GAA)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAPro(AGG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAPro(CGG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAPro(UGG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAThr(AGU)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAVal(AAC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAVal(CAC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine49 in tRNAVal(UAC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNA	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAAspGTC	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAGlu(CUC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAGlu(UUC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAGly precursor	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAGly(CCC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAGly(GCC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAGly(UCC)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAGlyGCC	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAHis	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNALeuCAA	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAPro(AGG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAPro(CGG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine50 in tRNAPro(UGG)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine59 in vtRNA1.2	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine59 in vtRNA1.3	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine69 in vtRNA1.1	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine72 in tRNACys	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine72 in tRNACys(GCA)	-	
2.1.1.202	S-adenosyl-L-methionine + cytosine72 in tRNAThr	-