1.14.19.6 (Z)-9-tetradecenoic acid + ? biosynthetic pathway for producing the sex pheromone component (Z,E)-9,12-tetradecadienyl acetate in moths involves a DELTA12 desaturase 1.14.19.6 1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ - 1.14.19.6 cis-9-octadecenoic acid + reduced acceptor + O2 PtFAD2 is involved in the biosynthesis of eicosapentaenoic acid 1.14.19.6 eicosenoic acid + reduced acceptor + O2 62% conversion 1.14.19.6 additional information oxygen availability alone can regulate de novo DELTA12-desaturase synthesis in Acanthamoeba castellanii and oxygen can limit the activity of preexisting DELTA12-desaturase 1.14.19.6 additional information PtFAD6 is involved in the biosynthesis of hexadecantrienic acid 1.14.19.6 additional information synergistic effect of high-light and low temperature on cell growth of the DELTA12 fatty acid desaturase mutant 1.14.19.6 additional information the main transition in fatty acid metabolism of Acanthamoeba castellanii during batch growth appears to be primarily related to a rapid decline in DELTA12-desaturase activity after 24 h. The resultant large growth-dependent changes in the degree of fatty acid unsaturation would be expected to affect the physical state and/or fluidity of membranes, and may be related to many of the distinctive physiological and biochemical characteristics displayed by Acanthamoeba castellanii in different stages of batch growth 1.14.19.6 oleic acid + ? - 1.14.19.6 oleic acid + ? DELTA12-desaturase mutant shows delayed spore germination, a twofold reduction in growth, a reduced level of conidiation and complete loss of sclerotial development, compared with the wild-type enzyme 1.14.19.6 oleic acid + ? expression in Saccharomyces cerevisiae leads to endogenous production of linoleic acid 1.14.19.6 oleic acid + ? linoleic acid was detectable when expressing Fm2 in DELTA12 desaturase knockout Yarrowia lipolytica 1.14.19.6 oleic acid + reduced acceptor + O2 specific substrate 1.14.19.6 oleic acid + reduced acceptor + O2 best substrate with 23% conversion 1.14.19.6 oleic acid + reduced acceptor + O2 best substrate with 63.3% conversion 1.14.19.6 oleic acid + reduced acceptor + O2 best substrate with 72% conversion 1.14.19.6 oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ - 1.14.19.6 oleoyl-CoA + 2 reduced acceptor + O2 + 2 H+ - 1.14.19.6 oleoyl-CoA + reduced acceptor + O2 the DELTA12 desaturase provides the key step for the cockroach to become nutritionally independent of dietary lipid and to synthesize eicosanoids de novo 1.14.19.6 oleoyl-CoA + reduced acceptor + O2 - 1.14.19.6 palmitoleic acid + reduced acceptor + O2 43% conversion 1.14.19.6 palmitoleic acid + reduced acceptor + O2 26.7% conversion 1.14.19.6 palmitoleic acid + reduced acceptor + O2 4.1% conversion 1.14.19.6 palmitoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ - 1.14.19.6 stearolic acid + reduced acceptor + O2 4.8% conversion 1.14.19.6 stearolic acid + reduced acceptor + O2 35% conversion