1.1.99.21	D-glucitol + NAD+	-	
1.1.99.21	D-sorbitol + acceptor	-	
1.1.99.21	D-sorbitol + acceptor	high specificity	
1.1.99.21	D-sorbitol + acceptor	D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase	
1.1.99.21	D-sorbitol + NADP+	-	
1.1.99.21	L-glucitol + NAD+	90% conversion	
1.1.99.21	additional information	production of 5-keto-D-gluconate is solely dependent on enzyme	
1.1.99.21	additional information	this SLDH is distinguished from other L-sorbose-producing enzymes by its high activity and substrate specificity. Isothermal titration calorimetry shows that the protein binds more strongly to D-sorbitol than other L-sorbose-producing enzymes, and substrate docking analysis confirms a higher turnover rate