3.4.24.74 additional information - analysis of BFT-2 by native PAGE reveals bands at 36000 Da, 90000 Da and 200000 Da. Both the 36000 Da and higher-molecular-mass bands are biologically active when tested in the HT29/C1 cell assay 651483 3.4.24.74 18000 22000 gel fitration 31497 3.4.24.74 19000 - 1 * 19000, SDS-PAGE 31497 3.4.24.74 20000 - gel electrophoresis and Western blot analysis, mature processed BFT in all mutants 668924 3.4.24.74 20000 - Western blot analysis of cell-free culture supernatants of strain NCTC 9343 expressing mutants BFT-C2, BFT-C4 and BFT-A394E, and of whole cell lysates in preperations of strain NCTC 9343 expressing wild type BFT 668932 3.4.24.74 20000 - x * 20000, SDS-PAGE 733433 3.4.24.74 20000 - x * 44000, preenzyme, x * 20000, mature enzyme 683384 3.4.24.74 20600 - 1 * 20600, SDS-PAGE 31496 3.4.24.74 20600 - cleaved to its enzymatically active secreted form 668836 3.4.24.74 21000 - x * 21000, SDS-PAGE 651475, 720923