2.5.1.78 additional information - the protein is an icosahedral capsid of 60 subunits with a mass of about 1 MDa as shown by hydrodynamic studies and by electron microscopy. The lumazine synthase of Escherichia coli is not physically associated with another enzyme of the riboflavin pathway, and the core of the icosahedral capsid is empty 698702 2.5.1.78 15500 - 60 * 15500, subunit of mature enzyme, SDS-PAGE 698712 2.5.1.78 15645 - 60 * 15645, the enzyme can form capsids with icosahedral 532 symmetry consisting of 60 subunits, electrospray mass spectrometry 697706 2.5.1.78 16000 - 60 * 16000, the enzyme can form capsids with icosahedral 532 symmetry consisting of 60 subunits, SDS-PAGE 697706 2.5.1.78 16008 - x * 16008, calculated, x * 17000-18000, SDS-PAGE, x * 18100, MALDI-TOF 721157 2.5.1.78 16156 - 60 * 16156, calculated from sequence 698702 2.5.1.78 16534 - 60 * 16534, subunit of mature enzyme, calculated from sequence 698712 2.5.1.78 16536 - 60 * 16536, subunit of mature enzyme, electrospray ionization mass spectrometry 698712 2.5.1.78 16800 - 5 * 16800, calculated, isoform RibH1 688382 2.5.1.78 17188 - 5 * 17188, calculated from sequence 697704 2.5.1.78 17189 - 5 * 17189, electrospray MS 697704 2.5.1.78 17200 - 5 * 17200, calculated, isoform RibH1 688382 2.5.1.78 17300 - 10 * 17300, calculated, isoform RibH2 688382 2.5.1.78 17599 - 5 * 17599, calculated from sequence 698574 2.5.1.78 18000 - 10 * 18000, it is demonstrated by means of solution light scattering and X-ray structural analyses that the enzyme assembles as a very stable dimer of pentamers. A mechanism for dissociation/unfolding of this macromolecular assembly is postulated 698729 2.5.1.78 18000 - x * 18000, SDS-PAGE 699478 2.5.1.78 18100 - x * 16008, calculated, x * 17000-18000, SDS-PAGE, x * 18100, MALDI-TOF 721157 2.5.1.78 18598 - 5 * 18598, calculated from sequence 698702 2.5.1.78 18600 - 5 * 18600, calculated from sequence 698698 2.5.1.78 19000 - x * 19000, calculated from sequence 700019 2.5.1.78 23230 - x * 23230, calculated 722434 2.5.1.78 25070 - - 739125 2.5.1.78 25410 - - 739125 2.5.1.78 75300 - isoform RibH1, light scattering experiments 688382 2.5.1.78 80100 - light scattering experiments, isoform RibH1 688382 2.5.1.78 85500 - isoform RibH1, light scattering experiments 688382 2.5.1.78 87000 - sedimentation equilibrium centrifugation 697704 2.5.1.78 88000 - sedimentation equilibrium experiments 698574 2.5.1.78 90000 - gel filtration 699478 2.5.1.78 90000 - sedimentation equilibrium analysis 698702 2.5.1.78 97770 - MALDI-TOF mass spectromety 737337 2.5.1.78 158500 - isoform RinH2, light scattering experiments 688382 2.5.1.78 234000 - recombinant chimera consisting of the B subunit of Shiga toxin type 2 and Brucella sp. lumazine synthase, i.e. BLS-Stx2B chimera, gel filtration 738788 2.5.1.78 850000 - gel filtration 698712 2.5.1.78 973000 991000 sedimentaion equilibrium studies 698712 2.5.1.78 977000 - sedimentation equilibrium analysis 698702