3.4.24.B6 Ca2+ dependent on 652736, 686743 3.4.24.B6 Ca2+ required 712555, 712586, 712590 3.4.24.B6 additional information neither calcium nor magnesium alone can effectively activate the proenzyme 684397 3.4.24.B6 Zn2+ - 684767 3.4.24.B6 Zn2+ 1 mM, activates effectively 684397 3.4.24.B6 Zn2+ dependent on 651269, 652736, 686743 3.4.24.B6 Zn2+ enzyme contains a zinc-binding site in the catalytic domain with the consensus sequence HEXGHXXGXXH at positions 226-236 649826 3.4.24.B6 Zn2+ part of the catalytic subdomain 686936