2.8.2.20 Ca2+ activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+ 645820 2.8.2.20 Co2+ 10 mM, stimulates 657791 2.8.2.20 Co2+ 20 mM, stimulates 658932 2.8.2.20 Co2+ 25% of the stimulation with Mn2+, 20 mM 645805 2.8.2.20 Cu2+ activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+ 645820 2.8.2.20 divalent cations requirement 645803 2.8.2.20 Mg2+ 1.2fold at 5 mM 645818 2.8.2.20 Mg2+ activates isozyme TPST-2 674372 2.8.2.20 Mg2+ activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+ 645820 2.8.2.20 Mg2+ exhibits around 50% of the stimulation attained with Mn2+ 658932 2.8.2.20 Mg2+ required 760527 2.8.2.20 Mg2+ requirement 645808, 645816 2.8.2.20 Mg2+ requirement for sulfation of tert-butoxycarbonylcholecystokinin 645816 2.8.2.20 Mn2+ - 645818 2.8.2.20 Mn2+ 20 mM 645805, 645828 2.8.2.20 Mn2+ 20 mM, 2.5fold stimulation 657791 2.8.2.20 Mn2+ 30 mM 645815 2.8.2.20 Mn2+ 5 mM 645810 2.8.2.20 Mn2+ activates isozymes TPST-1 674372 2.8.2.20 Mn2+ activates, optimal at 20 mM, not essential 676996 2.8.2.20 Mn2+ activation 645810, 645812, 645814 2.8.2.20 Mn2+ activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+ 645820 2.8.2.20 Mn2+ inhibits above 30 mM 645805 2.8.2.20 Mn2+ optimal concentration: 20 mM 645826 2.8.2.20 Mn2+ requirement 645805, 645807, 645815, 645816, 645828 2.8.2.20 MnCl2 required, maximal activity at 20 mM 658932 2.8.2.20 additional information Mg2+ cannot replace Mn2+ 645828 2.8.2.20 additional information no activation by Ca2+, Cd2+, Cu2+ or Zn2+ 645805 2.8.2.20 additional information no activation of isozyme TPST-1 by Mg2+ 674372 2.8.2.20 additional information no activation of isozyme TPST-2 by Mn2+ 674372 2.8.2.20 additional information no requirement for Co2+ 645815 2.8.2.20 additional information no requirement of divalent metal ions 737434 2.8.2.20 additional information no requirement of Mg2+ 645805 2.8.2.20 NaCl activation, sulfation of tert-butoxycarbonylcholecystokinin 645816 2.8.2.20 NaF - 645812, 645814 2.8.2.20 NaF increases product formation 10fold by preventing degradation of 3'-phosphoadenylylsulfate 645805 2.8.2.20 Zn2+ activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+ 645820