2.7.8.13	Mg2+	-	645332, 645335	
2.7.8.13	Mg2+	absolute requirement for divalent cations, optimal activity with Mg2+ at concentrations 40-75 mM	662239	
2.7.8.13	Mg2+	absolute requirement, maximun activity at 30-40 mM	645334	
2.7.8.13	Mg2+	can be replaced only by Mn2+	645340	
2.7.8.13	Mg2+	catalytic activity is dependent on Mg2+	725566	
2.7.8.13	Mg2+	essentially required	737788	
2.7.8.13	Mg2+	optimum concentration for wild-type activity: 30-60 mM, inhibitory above 100 mM, 2% of optimum activity left at 250 mM for wild-type and 30-70% for mutants (except mutant E299Q)	690996	
2.7.8.13	Mg2+	required	737580	
2.7.8.13	Mg2+	required for maximal activation, optimum concentration 25 mM	645336	
2.7.8.13	Mn2+	can substitute for Mg2+	645334	
2.7.8.13	Mn2+	enzyme activity in presence of Mn2+ is 1% of the activity detected in presence of Mg2+	662239	
2.7.8.13	additional information	Cu2+, Ca2+ and Zn2+ are without effect	645334	
2.7.8.13	additional information	no activity is observed using Ni2+, Ca2+ and Zn2+	645340