2.7.2.1 Ca2+ - 487537, 642172, 642173, 642181, 642184, 642189, 642196 2.7.2.1 Ca2+ can replace Mg2+, but less effectively 642178 2.7.2.1 Ca2+ isoenzyme I 642179 2.7.2.1 Ca2+ the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity 727804 2.7.2.1 Co2+ - 487537, 642172, 642179, 642181, 642184, 642189 2.7.2.1 Co2+ can partly substitute for Mn2+ or Mg2+ 737465 2.7.2.1 Co2+ can replace Mg2+ 642173, 642178 2.7.2.1 Co2+ the activity is strongly dependent on Mg2+ (100%), Mn2+ (100%) or Co2+ (68 %) ions 746769 2.7.2.1 Co2+ the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity 727804 2.7.2.1 Co2+ Vmax: 15%, compared to Mg2+ or Mn2+ 721949 2.7.2.1 Cu2+ - 642179 2.7.2.1 K+ the enzyme requires monovalent K+ and divalent Mn2+or Mg2+ cations 738528 2.7.2.1 Mg2+ - 487537, 642167, 642168, 642169, 642172, 642173, 642176, 642178, 642179, 642180, 642181, 642190, 642195, 642196, 642197 2.7.2.1 Mg2+ 1.3 mM used in assay conditions. The activity of the enzyme in the presence of Mg2+ is about 80% of that in the presence of Mn2+ 757471 2.7.2.1 Mg2+ 10 mM used in assay conditions 761006 2.7.2.1 Mg2+ 40 mM used in assay conditions 744070 2.7.2.1 Mg2+ 5 mM used in assay conditions 762441 2.7.2.1 Mg2+ absolute requirement 642183 2.7.2.1 Mg2+ absolute requirement at pH 7.4 642166 2.7.2.1 Mg2+ binding constants of enzyme variants with ADP measured 690888 2.7.2.1 Mg2+ dependence on Mg2+ or Mn2+ ions, Km values is 0.28 mM 737465 2.7.2.1 Mg2+ required 738717, 738854, 738863, 739218, 739591 2.7.2.1 Mg2+ required for activity 761931 2.7.2.1 Mg2+ strict requirement 642187 2.7.2.1 Mg2+ the activity is strongly dependent on Mg2+ (100%), Mn2+ (100%) or Co2+ (68 %) ions 746769 2.7.2.1 Mg2+ the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity 727804 2.7.2.1 Mg2+ the enzyme requires Mn2+ or Mg2+ 739591 2.7.2.1 Mg2+ the enzyme requires monovalent K+ and divalent Mn2+or Mg2+ cations 738528 2.7.2.1 Mg2+ Vmax: 100% 721949 2.7.2.1 Mn2+ - 487537, 642172, 642173, 642179, 642180, 642181, 642183, 642184, 642187, 642189, 642190, 642195, 642196 2.7.2.1 Mn2+ 10 mM used in assay conditions. The enzyme exhibits about 25% higher specific activity with manganese as a cofactor compared to magnesium 760452 2.7.2.1 Mn2+ dependence on Mg2+ or Mn2+ ions, Km values is 0.47 mM 737465 2.7.2.1 Mn2+ Mn2+ supports highest enzyme activity 757471 2.7.2.1 Mn2+ the activity is strongly dependent on Mg2+ (100%), Mn2+ (100%) or Co2+ (68 %) ions 746769 2.7.2.1 Mn2+ the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). The maximum rate is obtained with Mn2+ 727804 2.7.2.1 Mn2+ the enzyme requires Mn2+ or Mg2+ 739591 2.7.2.1 Mn2+ the enzyme requires monovalent K+ and divalent Mn2+or Mg2+ cations 738528 2.7.2.1 Mn2+ Vmax: 100% 721949 2.7.2.1 additional information Co2+, Ca2+, Cd2+ and Zn2+ can replace Mg2+ or Mn2+ only partially 642180 2.7.2.1 additional information no activity with Ba2+, Ca2+, Zn2+, or Cu2+ 737465 2.7.2.1 additional information no activity with Cu2+, Ni2+ or Zn2+ 642184 2.7.2.1 additional information no activity with Cu2+, Ni2+, Hg2+, Ba2+ or Fe2+ 642181 2.7.2.1 additional information no activity with Zn2+ 642189 2.7.2.1 Ni2+ - 487537, 642172 2.7.2.1 Ni2+ Vmax: 5%, compared to Mg2+ or Mn2+ 721949 2.7.2.1 Pb2+ - 642173 2.7.2.1 Zn2+ - 487537, 642172, 642179, 642181