2.3.2.B11	Ca2+	required	
2.3.2.B11	Ca2+	optimal concentration: 0.1 mM	
2.3.2.B11	Ca2+	the activity is regulated by the binding of the second messenger Ca2+ to the substrate calmodulin, which increases the activity ca. 10fold	
2.3.2.B11	Ca2+	during purification on ubiquitin-Sepharose the Ca2+-dependent activity is lost and an essentially Ca2+-independent enzyme is obtained which is purified 90fold	
2.3.2.B11	Ca2+	Km: 0.005-0.010 mM	
2.3.2.B11	Ca2+	Asr1p is involved in calcium signalling. The interaction with calmodulin occurs in vivo and depends on physiological concentrations of Ca2+. A plataeu is reached at 100-500 nM CaCl2