2.1.1.267	Ca2+	activates, less effective compared to Mg2+	757323	
2.1.1.267	Ca2+	interactions between flavonoid and enzyme are enhanced by metal-mediated electrostatic interactions in substrate binding site. Ca2+ and Mg2+ show similar effects	697062	
2.1.1.267	Ca2+	isoform ROMT-15, poorly substitutes for Mg2+	700855	
2.1.1.267	Ca2+	isoform ROMT-17, poorly substitutes for Mg2+	700855	
2.1.1.267	Co2+	activates, less effective compared to Mg2+	757323	
2.1.1.267	Co2+	isoform ROMT-15, may substitute for Mg2+	700855	
2.1.1.267	Co2+	isoform ROMT-17, may substitute for Mg2+	700855	
2.1.1.267	Co2+	the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Co2+ shows 41% of the activity with Mg2+ (with quercetin as substrate)	700855	
2.1.1.267	Co2+	the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Co2+ shows 82% of the activity with Mg2+ (with quercetin as substrate)	700855	
2.1.1.267	Mg2+	best activating cation, optimal at 1 mM	757323	
2.1.1.267	Mg2+	interactions between flavonoid and enzyme are enhanced by metal-mediated electrostatic interactions in substrate binding site. Ca2+ and Mg2+ show similar effects	697062	
2.1.1.267	Mg2+	isoform ROMT-15, required	700855	
2.1.1.267	Mg2+	isoform ROMT-17, required	700855	
2.1.1.267	Mg2+	the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity (with quercetin as substrate)	700855	
2.1.1.267	Mn2+	activates, less effective compared to Mg2+	757323	
2.1.1.267	Mn2+	the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Mn2+ shows 69% of the activity with Mg2+ (with quercetin as substrate)	700855	
2.1.1.267	Mn2+	the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Mn2+ shows 74% of the activity with Mg2+ (with quercetin as substrate)	700855	
2.1.1.267	additional information	the enzyme is an Mg2+-independent O-methyltransferase	756307	
2.1.1.267	Zn2+	activates, less effective compared to Mg2+	757323	
2.1.1.267	Zn2+	isoform ROMT-15, poorly substitutes for Mg2+	700855	
2.1.1.267	Zn2+	isoform ROMT-17, poorly substitutes for Mg2+	700855