1.1.1.90 Ag+ 0.01 mM, residual activity: 0.2% 724027 1.1.1.90 CaCl2 10 mM, 1.3fold increase in activity 692723 1.1.1.90 CaCl2 increases the enzyme activity by 27% at 10 mM 692723 1.1.1.90 CdCl2 10 mM, 1.3fold increase in activity 692723 1.1.1.90 CdCl2 increases the enzyme activity by 25% at 10 mM 692723 1.1.1.90 Cu2+ 0.1 mM, residual activity: 1.3% 724027 1.1.1.90 Fe2+ 1 mM, residual activity: 21.7% 724027 1.1.1.90 Hg+ 0.001 mM, residual activity: 0.1% 724027 1.1.1.90 KBr - 389581 1.1.1.90 KBr 10 mM, 1.1fold increase in activity 692723 1.1.1.90 KBr activity nearly doubled in presence of salts 389594 1.1.1.90 KBr increases the enzyme activity by 11% at 10 mM 692723 1.1.1.90 KCl - 389594 1.1.1.90 KCl activity nearly doubled 389581 1.1.1.90 KCl activity nearly doubled in presence of salts 389594 1.1.1.90 KNO3 - 389581 1.1.1.90 KNO3 activity nearly doubled in presence of salts 389594 1.1.1.90 Mg2+ - 667256 1.1.1.90 Mg2+ 1 mM, activity enhanced to 128% 724027 1.1.1.90 MgCl2 10 mM, 1.2fold increase in activity 692723 1.1.1.90 MgCl2 increases the enzyme activity by 22% at 10 mM 692723 1.1.1.90 MgCl2 slight activation 389581 1.1.1.90 Mn2+ 1 mM, activity enhanced to 128% 724027 1.1.1.90 additional information no effects on the enzyme activity by KCl, NaCl, and NiCl2, and by 10 mM urea and Tween-80 692723 1.1.1.90 NaCl - 389581 1.1.1.90 NaCl activity nearly doubled in presence of salts 389594 1.1.1.90 NH4Cl - 389581 1.1.1.90 NH4Cl activity nearly doubled in presence of salts 389594 1.1.1.90 Zn zinc-dependent alcohol dehydrogenase. All of the residues involved in zinc binding are conserved 692723 1.1.1.90 Zn2+ 1 mM, residual activity: 1.2% 724027 1.1.1.90 Zn2+ 1.9 mol of Zn2+ per mol subunit 389594 1.1.1.90 Zn2+ dependent on 735498 1.1.1.90 Zn2+ dependent on, a catalytic zinc atom bound at the active site to the ligands Cys46, Asp49, His67, and Cys174 and a structural zinc atom bound to cysteine residues 97, 100, 103, and 111. All of the residues involved in zinc binding are conserved 692723 1.1.1.90 Zn2+ required 739587