4.6.1.24 extracellular - - 24192, 24211, 24212, 24214, 683260, 730263, 730804 4.6.1.24 extracellular the enzyme is secreted - 717597 4.6.1.24 membrane - 16020 654991 4.6.1.24 additional information 99% of binase is present outside of the cell and only below 1% is localized intracellularly - 717597 4.6.1.24 additional information about 30–40% of barnase can be stored inside the cells for up to 90 min in complex with inhibitor barstar - 717597 4.6.1.24 additional information RNase Po1 might bind to the plasma membrane electrostatically - 729351 4.6.1.24 vacuole in contrast to the wild-type strain in which the inactivated RNase T1-GFP fusion protein is localized at the vacuole only under cold stress or nitrogen starvation, the inactivated RNase T1-GFP fusion protein expressed in the rns4 mutant is localized at the ER and vacuole, both under normal growth conditions and upon ambient stress conditions 5773 665007