3.1.3.36 cell wall - 5618 666576 3.1.3.36 clathrin-coated vesicle multicolor total internal reflection fluorescence microscopy (TIRFM) is used to visualize the spatial-temporal recruitment of SJ1, and its binding partner endophilin to clathrin-coated-pits. Differential temporal recruitment of the two major SJ1 splice variants to clathrin-coated-pits is observed. SJ1-145 is rapidly recruited as a burst, together with its binding partner endophilin, at a late stage of clathrin-coated-pit formation 30136 682526 3.1.3.36 clathrin-coated vesicle multicolor total internal reflection fluorescence microscopy (TIRFM) is used to visualize the spatialtemporal recruitment of SJ1, and its binding partner endophilin to clathrin-coated-pits. Differential temporal recruitment of the two major SJ1 splice variants to clathrin-coated-pits is observed. SJ1-170 is present at all stages of CCP formation 30136 682526 3.1.3.36 cytoplasm - 5737 678074, 681038 3.1.3.36 cytoskeleton - 5856 134805 3.1.3.36 cytosol - 5829 134805, 134807, 134808, 134810, 134811, 732476 3.1.3.36 cytosol preferential localization of the Mg2+-dependent enzyme in cytosol 5829 134808 3.1.3.36 cytosol SHIP2 is predominately cytosolic but translocates to the cell periphery in response to bpV(phen) 5829 678074 3.1.3.36 endoplasmic reticulum SKIP localizes to the ER in HuH-7 cells 5783 691698 3.1.3.36 endosome - 5768 751176 3.1.3.36 endosome dOCRL is associated with endosomes 5768 714782 3.1.3.36 Golgi apparatus - 5794 134815, 134821, 693264 3.1.3.36 Golgi apparatus subcellular localisation found in Golgi apparatus and ER-to-Golgi intermediate compartment (ERGIC). INPP5B binds to specific RAB proteins within the secretory pathway, and mutational analysis indicates that RAB binding is required for efficient Golgi targeting of INPP5B 5794 681014 3.1.3.36 Golgi membrane cytoplasmic Golgi membrane 139 652022 3.1.3.36 lysosome enzyme is associated with the lysosomal membrane 5764 134820 3.1.3.36 membrane - 16020 -, 134805, 134807, 134808, 134809, 713723, 716345, 716657 3.1.3.36 membrane dOCRL dephosphorylates PI(4,5)P2 on internal membranes 16020 714782 3.1.3.36 membrane upon insulin stimulation, the adaptor protein APS and SHIP2 are recruited to cell membranes as seen by immunofluorescence studies 16020 681038 3.1.3.36 membrane voltage-sensitive phosphoinositide phosphatase Ci-VSP 16020 716022 3.1.3.36 microsome - - 134816 3.1.3.36 additional information 77% of the activity is localized in the particulate fraction - 134812 3.1.3.36 additional information OCRL1a is significantly more abundant in the cytoplasmic puncta, and there is less diffuse cytosolic staining compared to isoform b in NRK- and HeLa cells - 693264 3.1.3.36 additional information particulate fraction - 134817 3.1.3.36 nucleus - 5634 751562 3.1.3.36 nucleus in HuH-7 cells overexpressing SKIP and infected with hepatitis B virus (HBV), SKIP localizes to nucleus in addition to endoplasmic reticulum and suppresses HBV gene expression and replication. SKIP loses its nuclear localization and suppressive effect during replication of a core-negative HBV mutant 5634 691698 3.1.3.36 outer membrane - 19867 134814 3.1.3.36 perinuclear space - - 678074 3.1.3.36 perinuclear space both isoforms a and b predominantly localize to the perinuclear region - 693264 3.1.3.36 plasma membrane - 5886 693056, 716164, 716345, 716921 3.1.3.36 plasma membrane CD32a cross-linking induces translocation to the plasma membrane 5886 679145 3.1.3.36 plasma membrane localization of SHIP2 at clathrin-coated pits is mediated by its proline-rich region. When the targeting signal present in this domain is missing, another targeting signal that directs the protein to focal adhesion predominates, overview 5886 715707 3.1.3.36 plasma membrane SHIP1 is localized to the membrane during cell adhesion 5886 732476 3.1.3.36 vacuole Dd5P4 accumulates on Legionella-containing vacuoles in wild-type Legionella pneumophilia infected Dictyostelium discoideum 5773 691699 3.1.3.36 vacuole in macrophages infected with Legionella pneumophila the localization of endogenous OCRL1 is identified on Legionella-containing vacuoles containing single or several bacteria 5773 691699 3.1.3.36 vacuole OCRL1 accumulates on Legionella-containing vacuoles in wild-type Legionella pneumophilia infected Dictyostelium discoideum 5773 691699