4.1.3.43	additional information	-	additional information	presence of NAD+, NADP+, NADH or CoA leads to an at least 5fold reduction in the Km for 4-hydroxy-2-oxopentanoate	721615	
4.1.3.43	additional information	-	additional information	value not detectable for R16A variant of BphI	714244	
4.1.3.43	additional information	-	additional information	with 4-hydroxy-2-oxopentanoate at 50°C Km-value is not affected	721659	
4.1.3.43	0.012	-	(4R)-4-hydroxy-2-oxopentanoate	Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.013	-	(4R)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.013	-	(4R)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.013	-	(4S)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.013	-	(4S)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.013	-	(4S)-4-hydroxy-2-oxopentanoate	Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.037	-	4-hydroxy-2-oxohexanoate	TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659