6.3.4.2 additional information - additional information kinetic mechanism, activation and inhibition kinetics 674909 6.3.4.2 additional information - additional information kinetics of wild-type and mutants 690817 6.3.4.2 additional information - additional information maximally active at physiological concentrations of ATP, GTP, and glutamine, whereas the Km and IC50 values for the substrate UTP and the product CTP, respectively, are close to their physiological concentrations 715549 6.3.4.2 additional information - additional information positive cooperativity for ATP and UTP 702613 6.3.4.2 0.002 - ATP - 674816 6.3.4.2 0.027 - L-Gln wild-type, pH 8.1, 37°C 715549 6.3.4.2 0.027 - UTP pH 8.0, 25°C, without GTP 648997 6.3.4.2 0.04 - UTP pH 8.0, 30°C, phosphorylated enzyme, in presence of 2 mM ATP 648994 6.3.4.2 0.05 - UTP pH 8.0, 30°C, native enzyme, in presence of 2 mM ATP 648994 6.3.4.2 0.054 - ATP pH 8.0, 25°C, without GTP 648997 6.3.4.2 0.063 - Gln-OH 37°C, pH 8.0, mutant enzyme L109A 661644 6.3.4.2 0.07 - ATP - 674786 6.3.4.2 0.07 - GTP - 648988, 648990, 674786 6.3.4.2 0.07 - UTP - 648988 6.3.4.2 0.07 - UTP pH 8.0, mutant enzyme S354A, kinetic constant determined with 0.5 mM ATP 648973 6.3.4.2 0.071 - UTP pH 8.0, 25°C, activation with GTP 648997 6.3.4.2 0.072 - L-Gln mutant S568A, pH 8.1, 37°C 715549 6.3.4.2 0.08 - UTP pH 8.0, 30°C, native enzyme or phosphorylated enzyme, in presence of 0.5 mM ATP 648994 6.3.4.2 0.097 - ATP pH 7.2 649000 6.3.4.2 0.1 - L-Gln wild-type, pH 8.1, 37°C 715549 6.3.4.2 0.1 - L-glutamine pH 7.4 37°C, crude extract 648990 6.3.4.2 0.1 - UTP pH 8.0, mutant enzyme S330A, kinetic constant determined with 0.5 mM ATP 648973 6.3.4.2 0.16 - UTP - 674786 6.3.4.2 0.165 - Gln-OH 37°C, pH 8.0, wild-type enzyme 661644 6.3.4.2 0.19 - UTP wild-type, pH 8.1, 37°C 715549 6.3.4.2 0.196 - glutamine mutant K306A, presence of 1 mM ATP, 1 mM UTP 672395 6.3.4.2 0.206 - glutamine wild-type protein, presence of 3 mM ATP, 2 mM UTP 672395 6.3.4.2 0.259 - Gln 30°C, pH 8.0 660794 6.3.4.2 0.26 - L-glutamine - 674786 6.3.4.2 0.345 - glutamine wild-type protein, presence of 1 mM ATP, 1 mM UTP 672395 6.3.4.2 0.354 - Gln 37°C, pH 8.0, wild-type enzyme 661644 6.3.4.2 0.42 - UTP mutant S568A, pH 8.1, 37°C 715549 6.3.4.2 0.424 - glutamine mutant K306A, presence of 3 mM ATP, 2 mM UTP 672395 6.3.4.2 0.497 - Gln 37°C, pH 8.0, mutant enzyme L109A 661644 6.3.4.2 0.59 - UTP wild-type, pH 8.1, 37°C 715549 6.3.4.2 0.627 - NH3 mutant K306A, presence of 1 mM ATP, 1 mM UTP 672395 6.3.4.2 0.8 - UTP mutant S571A, pH 8.1, 37°C 715549 6.3.4.2 1.47 - NH3 mutant K306A, presence of 3 mM ATP, 2 mM UTP 672395 6.3.4.2 1.54 - NH3 mutant K297A, presence of 1 mM ATP, 1 mM UTP 672395 6.3.4.2 1.9 - UTP C-terminal deletion mutant, pH 8.1, 37°C 715549 6.3.4.2 2.15 - NH3 37°C, pH 8.0, wild-type enzyme 661644 6.3.4.2 2.15 - NH3 wild-type protein, presence of 1 mM ATP, 1 mM UTP 672395 6.3.4.2 2.17 - NH3 37°C, pH 8.0, mutant enzyme L109A 661644 6.3.4.2 2.79 - NH3 wild-type protein, presence of 3 mM ATP, 2 mM UTP 672395 6.3.4.2 12.4 - UTP pH 8.0, 65°C 746547 6.3.4.2 39.4 - L-glutamine 37°C, pH 8.0, wild-type enzyme 661644 6.3.4.2 54 - NH4+ 30°C, pH 8.0, wild-type enzyme 661713 6.3.4.2 56 - NH4+ 30°C, pH 8.0, mutant enzyme G360A 661713 6.3.4.2 57 - NH4+ 30°C, pH 8.0, mutant enzyme R359M 661713 6.3.4.2 75.3 - NH2OH 37°C, pH 8.0, mutant enzyme L109A 661644 6.3.4.2 78 - NH4+ 30°C, pH 8.0, mutant enzyme E362Q 661713 6.3.4.2 82.8 - NH2OH 37°C, pH 8.0, wild-type enzyme 661644 6.3.4.2 92 - NH4+ 30°C, pH 8.0, mutant enzyme G360P 661713 6.3.4.2 92 - NH4+ 30°C, pH 8.0, mutant enzyme R359P 661713