6.2.1.1 additional information - additional information - 573 6.2.1.1 additional information - additional information kinetic parameters of wild type and mutant enzymes at 55°C, overview 693061 6.2.1.1 additional information - additional information kinetics and extent of reduction of the Fe4S4 cubane in the apo-alpha subunit and the Ni-activated a subunit upon exposure to titanium(III) citrate, detailed overview 687824 6.2.1.1 0.0037 - acetyl-CoA pH 7.5, 80°C, wild-type alpha-subunit 693061 6.2.1.1 0.0039 - acetyl-CoA pH 7.5, 55°C, wild-type enzyme 693061 6.2.1.1 0.0042 - acetyl-CoA pH 7.5, 80°C, wild-type enzyme 693061 6.2.1.1 0.011 - CoA - 652030 6.2.1.1 0.0139 - CoA pH 7.5, 55°C, wild-type enzyme 693061 6.2.1.1 0.0145 - CoA pH 7.5, 55°C 726837 6.2.1.1 0.017 - ATP in Tris buffer, pH 6.7, 200 mM K+ 563 6.2.1.1 0.02 - ATP pH 7.5, 55°C 726837 6.2.1.1 0.0224 - ATP pH 7.5, mutant enzyme R526A 672265 6.2.1.1 0.0238 - ATP pH 7.5, mutant enzyme A357V 672265 6.2.1.1 0.026 - acetyl-CoA pH 8.0 649463 6.2.1.1 0.0265 - ATP pH 7.5, mutant enzyme R584E 672265 6.2.1.1 0.0287 - ATP pH 7.5, mutant enzyme R194A 672265 6.2.1.1 0.035 - CoA - 554, 559 6.2.1.1 0.0373 - ATP pH 7.5, mutant enzyme R194E 672265 6.2.1.1 0.0381 - ATP pH 7.5, mutant enzyme R584A 672265 6.2.1.1 0.04 - acetate - 555 6.2.1.1 0.044 - ATP pH 7.5, mutant enzyme D517P 672265 6.2.1.1 0.048 - CoA - 569 6.2.1.1 0.05 - CoA pH 7.5, wild-type enzyme 672265 6.2.1.1 0.05 - CoA value above 0.05 mM 555 6.2.1.1 0.0638 - ATP pH 7.5, mutant enzyme G524S 672265 6.2.1.1 0.066 - acetyl-CoA - 580 6.2.1.1 0.073 - acetate - 652030 6.2.1.1 0.0771 - ATP pH 7.5, wild-type enzyme 672265 6.2.1.1 0.08 - CoA pH 7.0, 65°C, mutant enzyme G501A 726838 6.2.1.1 0.093 - ADP pH 7.5, 55°C, wild-type enzyme 693061 6.2.1.1 0.1 - ATP in Pipes buffer, pH 6.7, 200 mM K+ 563 6.2.1.1 0.1 - CoA pH 7.0, 65°C, mutant enzyme Y498A 726838 6.2.1.1 0.104 - CoA - 560 6.2.1.1 0.1072 - CoA pH 7.5, mutant enzyme R194E 672265 6.2.1.1 0.12 - CoA - 558 6.2.1.1 0.133 - CoA pH 7.5, mutant enzyme A357V 672265 6.2.1.1 0.14 - acetate CoA 572 6.2.1.1 0.14 - acetate pH 6.7, 200 mM K+ 563 6.2.1.1 0.1417 - CoA pH 7.5, mutant enzyme R194A 672265 6.2.1.1 0.146 - acetate - 568 6.2.1.1 0.16 - acetate - 572 6.2.1.1 0.16 - acetate pH 8.0 649463 6.2.1.1 0.16 - ATP synthesis of adenosine 5´-tetraphosphate 577 6.2.1.1 0.18 - CoA - 574 6.2.1.1 0.18 - CoA 65°C, mutant enzyme I312A 672066 6.2.1.1 0.18 - CoA 65°C, mutant enzyme V388G 672066 6.2.1.1 0.19 - CoA - 671665 6.2.1.1 0.19 - CoA 65°C, wild-type enzyme 672066 6.2.1.1 0.19 - CoA pH 7.0, 65°C, wild-type enzyme 726838 6.2.1.1 0.202 - CoA - 568 6.2.1.1 0.205 - CoA pH 7.5, mutant enzyme R526A 672265 6.2.1.1 0.208 - acetate - 566 6.2.1.1 0.21 - CoA - 563 6.2.1.1 0.22 - acetate CoA, in Tris buffer, pH 8.3, 200 mM K+ 563 6.2.1.1 0.221 - ATP pH 7.5, 55°C, wild-type enzyme 693061 6.2.1.1 0.228 - CoA pH 7.5, mutant enzyme D517G 672265 6.2.1.1 0.23 - phosphate pH 7.5, 80°C, wild-type enzyme 693061 6.2.1.1 0.238 - CoA - 566 6.2.1.1 0.24 - CoA pH 7.0, 65°C, mutant enzyme T499A 726838 6.2.1.1 0.243 - ATP pH 7.5, mutant enzyme D517G 672265 6.2.1.1 0.245 - ATP - 652030 6.2.1.1 0.25 - acetate - 560 6.2.1.1 0.25 - ATP - 572 6.2.1.1 0.25 - Sodium acetate isoform ACS1, at pH 7.8 and 25°C 746504 6.2.1.1 0.272 - phosphate pH 7.5, 55°C, wild-type enzyme 693061 6.2.1.1 0.275 - ATP - 568 6.2.1.1 0.28 - acetate - 554, 559 6.2.1.1 0.28 - CoA in Tris buffer, pH 6.7, 200 mM K+ 563 6.2.1.1 0.3 - CoA AF-ACS2 671665 6.2.1.1 0.35 - ATP Tris buffer, pH 8.3, 200 mM K+ 563 6.2.1.1 0.35 - CoA 65°C, mutant enzyme V388A 672066 6.2.1.1 0.3583 - CoA pH 7.5, mutant enzyme R584A 672265 6.2.1.1 0.36 - ATP - 560 6.2.1.1 0.37 - acetate - 553 6.2.1.1 0.4 - acetate pH 7.5, 55°C 726837 6.2.1.1 0.4 - ADP - 568 6.2.1.1 0.426 - CoA pH 7.5, mutant enzyme R584E 672265 6.2.1.1 0.43 - CoA 65°C, mutant enzyme W416G 672066 6.2.1.1 0.448 - CoA pH 7.5, mutant enzyme G524S 672265 6.2.1.1 0.46 - Butyrate 65°C, mutant enzyme T313V 672066 6.2.1.1 0.5 1 CoA pH 7.0, 65°C, mutant enzyme A500T 726838 6.2.1.1 0.513 - acetate - 550 6.2.1.1 0.52 - ATP pH 8.0 649463 6.2.1.1 0.527 - CoA pH 7.5, mutant enzyme D517P 672265 6.2.1.1 0.625 - acetate pH 7.5, 55°C, wild-type enzyme 693061 6.2.1.1 0.65 - acetate - 563 6.2.1.1 0.65 - ATP 65°C, mutant enzyme T313V 672066 6.2.1.1 0.7 - CoA 65°C, mutant enzyme T313V 672066 6.2.1.1 0.71 - ATP - 563 6.2.1.1 0.72 - Sodium acetate isoform ACS2, at pH 7.8 and 25°C 746504 6.2.1.1 0.86 - acetate - 569 6.2.1.1 1.2 - ATP - 554, 559 6.2.1.1 1.2 - CoA in Pipes buffer, pH 6.7, 200 mM K+ 563 6.2.1.1 1.4 - acetate pH 7.0, 65°C, mutant enzyme G501A 726838 6.2.1.1 1.7 - acetate AF-ACS2 671665 6.2.1.1 1.7 - ATP pH 7.0, 65°C, mutant enzyme G501A 726838 6.2.1.1 1.7 - ATP pH 7.0, 65°C, mutant enzyme T499A 726838 6.2.1.1 1.7 - ATP pH 7.0, 65°C, mutant enzyme Y498A 726838 6.2.1.1 1.8 - acetyl-CoA - 577 6.2.1.1 1.9 - ATP - 558 6.2.1.1 2.1 - acetate pH 7.0, 65°C, mutant enzyme A500T 726838 6.2.1.1 2.3 - ATP 65°C, mutant enzyme W416G 672066 6.2.1.1 2.3 - dATP - 568 6.2.1.1 2.6 - ATP 65°C, mutant enzyme V388A 672066 6.2.1.1 2.61 - acetate - 558 6.2.1.1 2.9 - acetate isoform Acs3, at pH 7.7 and 37°C 745789 6.2.1.1 2.9 - ATP AF-ACS2 671665 6.2.1.1 3 - acetate pH 7.0, 65°C, mutant enzyme T499A 726838 6.2.1.1 3.1 - propanoate - 553 6.2.1.1 3.3 - acetate isoform Acs2, at pH 7.7 and 37°C 745789 6.2.1.1 3.3 - ATP - 671665 6.2.1.1 3.3 - ATP 65°C, wild-type enzyme 672066 6.2.1.1 3.3 - ATP pH 7.0, 65°C, wild-type enzyme 726838 6.2.1.1 3.4 - ATP 65°C, mutant enzyme T313K 672066 6.2.1.1 3.5 - acetate - 671665 6.2.1.1 3.5 - acetate 65°C, wild-type enzyme 672066 6.2.1.1 3.5 - acetate pH 7.0, 65°C, wild-type enzyme 726838 6.2.1.1 3.59 - potassium acetate at pH 8.0 and 37°C 746449 6.2.1.1 3.6 - ATP pH 7.0, 65°C, mutant enzyme A500T 726838 6.2.1.1 3.9 - propionate AF-ACS2 671665 6.2.1.1 4 - ATP 65°C, mutant enzyme V388G 672066 6.2.1.1 4 10 acetate mutant L641P, pH not specified in the publication, temperature not specified in the publication 716267 6.2.1.1 4.1 - propionate 65°C, mutant enzyme V388A 672066 6.2.1.1 4.5 - acetate isoform Acs1, at pH 7.7 and 37°C 745789 6.2.1.1 4.5 - propionate 65°C, mutant enzyme T313V 672066 6.2.1.1 4.66 - Sodium acetate at pH 8.0 and 37°C 746449 6.2.1.1 4.7 - tripolyphosphate synthesis of adenosine 5´-tetraphosphate 577 6.2.1.1 5.26 - Acrylate - 553 6.2.1.1 5.7 - acetate - 557 6.2.1.1 6.1 - hexanoate 65°C, mutant enzyme W416G 672066 6.2.1.1 6.6 - ATP 65°C, mutant enzyme I312A 672066 6.2.1.1 6.8 - acetate - 574 6.2.1.1 6.8 - Butyrate - 557 6.2.1.1 7.5 - acetate pH 7.0, 65°C, mutant enzyme Y498A 726838 6.2.1.1 7.6 - 4-methylvalerate 65°C, mutant enzyme W416G 672066 6.2.1.1 8.3 - propanoate - 550 6.2.1.1 9 - propanoate - 560 6.2.1.1 10 - propanoate - 554, 559 6.2.1.1 10.2 - Heptanoate 65°C, mutant enzyme W416G 672066 6.2.1.1 10.5 - propanoate - 557 6.2.1.1 11.1 - valerate 65°C, mutant enzyme W416G 672066 6.2.1.1 13.2 - acetate 65°C, mutant enzyme V388A 672066 6.2.1.1 17 - Acrylate - 554, 559 6.2.1.1 17 - ATP - 574 6.2.1.1 18.1 - Octanoate 65°C, mutant enzyme W416G 672066 6.2.1.1 24.6 - acetate 65°C, mutant enzyme I312A 672066 6.2.1.1 29.2 - 2-methylvalerate 65°C, mutant enzyme W416G 672066 6.2.1.1 31.2 - fluoroacetate - 550 6.2.1.1 36.5 - propionate - 671665 6.2.1.1 36.5 - propionate 65°C, wild-type enzyme 672066 6.2.1.1 39.2 - Butyrate 65°C, mutant enzyme W416G 672066 6.2.1.1 41 - acetate wild-type Acs, pH not specified in the publication, temperature not specified in the publication 716267 6.2.1.1 128.6 - propionate 65°C, mutant enzyme V388G 672066 6.2.1.1 132.1 - acetate 65°C, mutant enzyme W416G 672066 6.2.1.1 133 - Butyrate AF-ACS2 671665 6.2.1.1 151.9 - Butyrate 65°C, mutant enzyme V388A 672066 6.2.1.1 164.4 - acetate 65°C, mutant enzyme V388G 672066 6.2.1.1 188.8 - propionate 65°C, mutant enzyme W416G 672066 6.2.1.1 596 - acetate 65°C, mutant enzyme T313K 672066 6.2.1.1 11000 - acetate mutant G266S, pH not specified in the publication, temperature not specified in the publication 716267