4.3.3.6 0.01 - D-ribose 5-phosphate pH 8.5, 37°C 748203 4.3.3.6 0.026 - D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.032 - D-ribose 5-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C 726269 4.3.3.6 0.035 - D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.04 - D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.04 - D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C 726269 4.3.3.6 0.043 - D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.043 - D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.044 - D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.068 - D-ribose 5-phosphate pH 8.0, 37°C 715478 4.3.3.6 0.077 - D-glyceraldehyde 3-phosphate pH 8.0, 37°C 715478 4.3.3.6 0.122 - D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.126 - D-ribose 5-phosphate pH 8.0, 37°C, wild-type enzyme 715491 4.3.3.6 0.155 - glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C 726269 4.3.3.6 0.185 - D-ribose 5-phosphate pH 8.0, 37°C, mutant enzyme K149R 715491 4.3.3.6 0.186 - glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C 726269 4.3.3.6 0.195 - glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.22 - glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.229 - glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.249 - glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.262 - glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.267 - glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.307 - glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.6 - L-glutamine pH 8.5, 37°C, glutaminase activity 748203 4.3.3.6 0.93 - L-glutamine Pdx2 glutaminase activity, R288K mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 0.99 - L-glutamine pH 8.0, 37°C 715478 4.3.3.6 1.06 - D-glyceraldehyde 3-phosphate pH 8.5, 37°C 748203 4.3.3.6 1.12 - L-glutamine Pdx2 glutaminase activity, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 1.16 - L-glutamine Pdx2 glutaminase activity, His-tagged wild-type protein, pH 7.5, 37°C 726269 4.3.3.6 1.16 - L-glutamine Pdx2 glutaminase activity, wild-type protein, pH 7.5, 37°C 726269 4.3.3.6 1.2 - L-glutamine Pdx2 glutaminase activity, R288A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 1.31 - L-glutamine Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 1.33 - L-glutamine Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 1.44 - L-glutamine Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C 726269 4.3.3.6 1.54 - L-glutamine Pdx2 glutaminase activity, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C 726269