4.1.3.43	additional information	-	additional information	presence of NAD+, NADP+, NADH or CoA leads to an at least 5fold reduction in the Km for 4-hydroxy-2-oxopentanoate	721615	
4.1.3.43	additional information	-	additional information	value not detectable for R16A variant of BphI	714244	
4.1.3.43	additional information	-	additional information	with 4-hydroxy-2-oxopentanoate at 50°C Km-value is not affected	721659	
4.1.3.43	0.012	-	(4R)-4-hydroxy-2-oxopentanoate	Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.013	-	(4R)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.013	-	(4R)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.013	-	(4S)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.013	-	(4S)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.013	-	(4S)-4-hydroxy-2-oxopentanoate	Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.037	-	4-hydroxy-2-oxohexanoate	TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.041	-	4-hydroxy-2-oxopentanoate	TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.045	-	4-hydroxy-2-oxopentanoate	app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	0.053	-	4-hydroxy-2-oxopentanoate	G322F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C	721651	
4.1.3.43	0.055	-	4-hydroxy-2-oxopentanoate	app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	0.06	-	4-hydroxy-2-oxopentanoate	G323A mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.074	-	4-hydroxy-2-oxopentanoate	app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	0.08	-	4-hydroxy-2-oxopentanoate	app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	0.081	-	4-hydroxy-2-oxopentanoate	G322L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C	721651	
4.1.3.43	0.089	-	(4S)-4-hydroxy-2-oxopentanoate	wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.089	-	(4S)-4-hydroxy-2-oxopentanoate	wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.089	-	4-hydroxy-2-oxopentanoate	BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.089	-	4-hydroxy-2-oxopentanoate	wild-type protein, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.09	-	4-hydroxy-2-oxopentanoate	G322F mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.095	-	4-hydroxy-2-oxopentanoate	app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	0.117	-	4-hydroxy-2-oxohexanoate	BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.117	-	4-hydroxy-2-oxohexanoate	wild-type protein, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.13	-	4-hydroxy-2-oxopentanoate	G323F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C	721651	
4.1.3.43	0.14	-	4-hydroxy-2-oxopentanoate	G322L mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.158	-	4-hydroxy-2-oxopentanoate	wild-type protein, steady state, absence of BphJ cofactors, pH 8.0, 25°C	721651	
4.1.3.43	0.16	-	4-hydroxy-2-oxopentanoate	G323L mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.18	-	4-hydroxy-2-oxohexanoate	pH 8.0, 25°C	721615	
4.1.3.43	0.18	-	4-hydroxy-2-oxopentanoate	G322A mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.199	-	4-hydroxy-2-oxohexanoate	BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.206	-	4-hydroxy-2-oxopentanoate	single TTHB246, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.21	-	4-hydroxy-2-oxohexanoate	single TTHB246, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.21	-	oxaloacetate	L87N variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.216	-	4-hydroxy-2-oxopentanoate	G323L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C	721651	
4.1.3.43	0.218	-	4-hydroxy-2-oxohexanoate	TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.22	-	4-hydroxy-2-oxopentanoate	pH 8.0, 25°C	721615	
4.1.3.43	0.222	-	(4S)-4-hydroxy-2-oxopentanoate	H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.228	-	(4S)-4-hydroxy-2-oxopentanoate	H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.257	-	4-hydroxy-2-oxopentanoate	TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.295	-	4-hydroxy-2-oxopentanoate	BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.3	-	4-hydroxy-2-oxohexanoate	G322F mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.35	-	4-hydroxy-2-oxoheptanoate	pH 8.0, 25°C	721615	
4.1.3.43	0.35	-	4-hydroxy-2-oxohexanoate	G322L mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.36	-	4-hydroxy-2-oxohexanoate	G323A mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.37	-	4-hydroxy-2-oxohexanoate	G323L mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.435	-	(4R)-4-hydroxy-2-oxopentanoate	L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.52	-	4-hydroxy-2-oxohexanoate	G322A mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	0.757	-	(4R)-4-hydroxy-2-oxopentanoate	L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.84	-	oxaloacetate	L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.92	-	oxaloacetate	Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.94	-	4-hydroxy-2-oxopentanoate	G323F mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	1.1	-	4-hydroxy-2-oxohexanoate	G323F mutant, steady state, pH 8.0, 25°C	721651	
4.1.3.43	6.2	-	oxaloacetate	L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	7.4	-	oxaloacetate	wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	7.5	-	oxaloacetate	pH 8.0, 25°C	721615	
4.1.3.43	11	-	pyruvate	L89A variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	13	-	pyruvate	wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	714244, 722458	
4.1.3.43	14.8	-	pyruvate	steady-state kinetic parameter, pH 8.0 and 25°C	702377	
4.1.3.43	16.8	-	propionaldehyde	L87A variant of BphI, app. Km-value, pH 8.0, 25°C	714244	
4.1.3.43	20	-	pyruvate	Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	20.2	-	pyruvate	L87A variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	24.4	-	propionaldehyde	L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	24.8	-	pentaldehyde	L89A variant of BphI, app. Km-value, pH 8.0, 25°C	714244	
4.1.3.43	30.2	-	pyruvate	L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	33.3	-	pentaldehyde	L87A/L89A variant of BphI, app. Km-value, pH 8.0, 25°C	714244	
4.1.3.43	38.2	-	pyruvate	L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	40.2	-	propionaldehyde	L89A variant of BphI, app. Km-value, pH 8.0, 25°C	714244	
4.1.3.43	49.2	-	Butyraldehyde	L89A variant of BphI, app. Km-value, pH 8.0, 25°C	714244	
4.1.3.43	54.9	-	propionaldehyde	L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	64.2	-	acetaldehyde	wild-type BphI, app. Km-value, pH 8.0, 25°C	714244	
4.1.3.43	64.2	-	acetaldehyde	wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	64.3	-	acetaldehyde	app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	67	-	acetaldehyde	steady-state kinetic parameter, pH 8.0 and 25°C	702377	
4.1.3.43	70.7	-	acetaldehyde	L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	80.5	-	acetaldehyde	L89A variant of BphI, app. Km-value, pH 8.0, 25°C	714244	
4.1.3.43	80.5	-	pentaldehyde	Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	81	-	acetaldehyde	Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	81.2	-	propionaldehyde	Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	86.3	-	acetaldehyde	L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	104	-	Butyraldehyde	Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	124.6	-	glycolaldehyde	app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	135.9	-	propionaldehyde	app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	136	-	propionaldehyde	wild-type BphI, app. Km-value, pH 8.0, 25°C	714244	
4.1.3.43	136	-	propionaldehyde	wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	289	-	Butyraldehyde	L87A/L89A variant of BphI, app. Km-value, pH 8.0, 25°C	714244	
4.1.3.43	323	-	Butyraldehyde	L87A variant of BphI, app. Km-value, pH 8.0, 25°C	714244